Four different simple, accurate, rapid, economical spectroscopic methods namely Zero order, First derivative, Area Under Curve and Three wavelength spectroscopy have been developed and validated for estimation of Pamabrom in tablet dosage form using Distilled Water as a solvent according to ICH Guidelines Q2R1. Absorbance of each solution was measured at 279.60 nm for zero order spectrophotometry. First derivative spectra are obtained by transformations at Δλ of 2 nm and scaling factor 10 and absorbance measured at 268.40 nm. Peak area is measured between 265.00-290.00 nm for AUC method. For three wavelength spectroscopy, absorbance was recorded at three wavelengths λ1= 254.60 nm, λ2 = 279.60 nm, λ3 = 294.00 nm. Pamabrom show linearity over the range of 4-24 μg/ml for all developed methods. Statistical comparisons by one way ANOVA shows all methods have equal applicability for estimation of Pamabrom in tablet dosage forms.
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